protocols

 

CRESYL VIOLET


Cresyl violet  stain (Nissl stain) stains all cells a blue/purple color

Cresyl violet stain .pdf


IMMUNOCYTOCHEMISTRY

Immunocytochemistry using rabbit polyclonal antibodies

Immunocytochemistry 1.pdf


Immunocytochemistry using mouse monoclonal antibodies

Immunocytochemistry 2.pdf


OTHER

Stock solutions has information to make buffers and fixative.

Stock solutions.pdf


Treated (subbed) slides are necessary so that mounted sections do not fall of the slide.

Treated Slides.doc


Storage solution (DeOlmos) allows antigenicity of fixed tissue to be preserved by freezing the sections. So they do not sustain damage the solution has a high concentration of sucrose and ethylene glycol which has a very low freezing point.

DeOlmos Solution for web-1.pdf


Procedures for resectioning hippocampal slices and processing after filling cells intracellularly with Neurobiotin or biocytin

Neurobiotin or Biocytin intracellular injection and processing.pdf